Characterize your microscope objective lenses, see how well matched they are to your microscope's image acquisition system. Input the magnification, immersion medium (air, oil, glycerol or silicone oil), NA (numerical aperture) and wavelength of light (lambda), to calculate the lateral resolution, axial resolution and fluorescence brightness of the objective. Characterize your acquisition system (camera pixel size, camera binning and additional magnification) to check if you're sampling at Nyquist frequency. Each of your objectives can be saved and easily restored, and shared with other microscopists via e-mail, MMS, Linked-In or even Facebook (why not?). The equations used for the calculations can be displayed by selecting the "Show Equations" option from the App bar menu. The sources of equations are referenced in the "About" box.

* Theoretical Resolution is calculated according Ernst Abbe's famous equation (Abbe 1873)