Prijepolje Museum was opened in 1990 with a complex museum collection. In 1995, it was granted the status of a regional museum covering the territories of the municipalities of Prijepolje and Priboj.

For 30 years the Prijepolje Museum has conducted major projects for the reconstruction of demolished medieval monasteries, reconstruction and protection of Islamic monuments, scientific research and reconnaissance of terrains, scientific meetings, lectures, museum exhibitions in the country and abroad and major visual arts projects. The project of reconstruction and extension of the museum building (2005-2007) with the National Investment Plan funds was designated a project of priority in the culture of Serbia.